Journal: BMC Veterinary Research

Article Title: Attenuated Actinobacillus pleuropneumoniae double-deletion mutant S-8 ∆clpP/apxIIC confers protection against homologous or heterologous strain challenge

doi: 10.1186/s12917-016-0928-9

Figure Lengend Snippet: Levels of IgG antibody ( a ), IgG1 ( b ), and IgG2 ( c ) in the sera of piglets. Blood samples were collected prior to and following immunization on days 0, 21, and 35 from the S-8 △clp△apxIIC immunized groups and PBS control groups, and the antibody responses were determined by indirect ELISA. The results are expressed as the means ± SD

Article Snippet: For determining the IgG isotypes, the sera were added to the S-8 △clpP△apxIIC -coated plates and incubated with mouse anti-pig IgG1 Monoclonal Antibody (Clone K139 3C8, Thermo, United Kingdom) or mouse anti-pig IgG2 Monoclonal Antibody (Clone K68 Ig2, Thermo, United Kingdom), followed by HRP-conjugated goat anti-mouse IgG (Abcam, United Kingdom).

Techniques: Control, Indirect ELISA

Journal: Nutrients

Article Title: Flavanol-Rich Cocoa Powder Interacts with Lactobacillus rhamnossus LGG to Alter the Antibody Response to Infection with the Parasitic Nematode Ascaris suum

doi: 10.3390/nu9101113

Figure Lengend Snippet: Anti- Ascaris IgG2 antibody response. The anti- Ascaris IgG2 antibody response was measured using A . suum proteins extracted from isolated third and fourth stage larvae and serum obtained 10 days post-inoculation with infective A . suum eggs. Bound serum antibody at a 1/1000 dilution was detected using biotinylated anti-pig IgG2 followed by streptavidin-HRP complex. Values shown are means ± SEM ( n = 8 per treatment). Labeled means without a common letter differ, p < 0.05.

Article Snippet: After six washes, 100 µL of HRP-conjugated porcine anti-IgM, IgG, IgG1 or IgG2 (Serotec, Raleigh, NC, USA) were added at a 1:10,000 dilution and incubated for one hour.

Techniques: Isolation, Labeling